protein analytics

We offer a range of protein analytic services to characterize the identity, purity, quantity and quality at every stage of your protein - from discovery all the way to manufacturing. Every protein purified at ATUM includes the following standard analytical package: appearance description, quantitation by A280, molecular weight and purity analysis by micro-capillary electrophoresis, and aggregation analysis by SEC-HPLC.

The standard package can be augmented with other protein analytical services. We can work with you to build an analytical package appropriate for your molecule and subsequent applications (e.g., IND filing, toxicology studies).

protein
analytics

We offer a range of protein analytic services to characterize the identity, purity, quantity and quality at every stage of your protein - from discovery all the way to manufacturing. Every protein purified at ATUM includes the following standard analytical package: appearance description, quantitation by A280, molecular weight and purity analysis by micro-capillary electrophoresis, and aggregation analysis by SEC-HPLC.

The standard package can be augmented with other protein analytical services. We can work with you to build an analytical package appropriate for your molecule and subsequent applications (e.g., IND filing, toxicology studies).

our protein analytics
offerings

Below is a menu of our current analytical offerings. Don't see what you need? Give us a call.

Protein Analytics

Protein Analytics Overview

Discovery/
Engineering

Titer measurements

  • HPLC
  • Octet
Epitope binning*
  • Octet
Identity & Purity
  • SEC-HPLC
  • µCE-SDS
  • MS Identity*
Hydrophobicity*
  • RP-HPLC
  • HIC-HPLC
Charge heterogeneity*
  • cIEX-HPLC
  • cIEF
Aggregation propensity*
  • AC-SINS
  • DLS (Hydrodynamic radius, kD, A2)
Thermostability
  • Tm (DSF)
  • Tagg (DLS)*
Polyspecificity
  • BVP-ELISA

Stability

  • pH stress
  • Thermal stress
  • Freeze-thaw stress
  • Agitation stress
Readout:
  • SEC-HPLC
  • µCE-SDS

Manufacturability*

  • Formulation study
  • Concentration study
Readout:
  • SEC-HPLC
  • µCE-SDS
  • DLS (Hydrodynamic radius, kD, A2)
  • HCP Analysis
  • HCPDNA Analysis
  • Glycan Analysis

Activity*

Binding kinetics

  • Octet, SPR
  • ELISA
Cell based Assays
FcgRI, FcRn interaction
  • Octet, SPR
Enzyme kinetics

* Enquire for more details

Developability Analytics Overview

See case studies on:
mAbs developability analytics
Bispecifics & Fc-fusion developability analytics
DLS

in silico predictions

  • Mol. wt.
  • Isoelectric point (pI)
  • N-Glycans
  • Hydrophobicity-GRAVY
  • Cysteine count
  • Sequence liabilities (deamidation, isomerization, acid labile)
  • Epitope analysis

Discovery/Engineering

Identity & Purity

  • SEC-HPLC
  • µCE-SDS
Aggregation propensity
  • AC-SINS
  • DLS (Hydrodynamic radius, kD, A2)*
Thermostability
  • Tm (DSF)
  • Tagg (DLS)*
Polyspecificity
  • BVP-ELISA

Stability

  • pH stress
  • Thermal stress
  • Freeze-thaw stress
  • Agitation stress
Readout:
  • SEC-HPLC
  • µCE-SDS

* Enquire for more details

Afucosylation Analytics Overview

See case studies on Afucosylation Analytics

Titer measurement (Harvest supernatant)

  • HPLC
  • Octet
Standard QC (purified protein)
  • SEC-HPLC
  • CE-SDS
Glycan analysis (purified protein)
  • HILIC-HPLC
Activity (purified protein)
  • ADCC (pseudo)
  • SPR binding

Bispecifics/Multispecifics Analytics Overview

See case studies on Bispecifics Analytics

Titer measurement (Harvest supernatant)

  • HPLC
  • Octet
Standard QC (purified protein)
  • SEC-HPLC
  • CE-SDS
Heterodimer % (purified protein)*
  • HIC-HPLC
  • cIEX-HPLC
Activity (purified protein)*
  • Octet
  • ELISA

* Enquire for more details

Have a question?
Let's talk.

ATUM customer support scientists are available to discuss cloning strategies, gene design constraints, bioinformatics analyses, and other molecular biology/biotechnology concerns.

Call

Corporate Headquarters
(Newark, California)
+1 650 853 8347

Email

We generally reply within a few hours.

info@atum.bio