DNA2.0

Design and Synthesis

ATUM is building on DNA2.0's reputation for rapid, reliable and accurate DNA synthesis. Using our proprietary GeneGPS® and VectorGPS® platforms we design constructs optimized to express in your system, whether that's a single gene in E. coli, a metabolic pathway in yeast or a complex bispecific antibody in mammalian cells.

Yield

We optimize expression constructs to maximize the yield of your protein from your expression host.

Control

Our inducible/repressible expression systems give you control of when your gene is expressed; localization sequences direct your protein to the correct intracellular compartment.

Precision

If you have a multi-chain protein such as an antibody or bispecific, we help you to precisely control the relative expression levels of each chain.

Developability

By solving expression issues early, we help smooth and accelerate your path through process development into manufacturing.

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Gene Optimization
Powered by GeneGPS®

Not all codon optimization algorithms are created equal. GeneGPS eliminates bias, incorrect assumptions and flawed optimization strategies by focusing on what matters - what has been experimentally demonstrated to improve expression!

GeneGPS begins with a training set of systematically varied synthetic gene variants, whose expression is experimentally tested in a target host. The expression data is analyzed using machine learning to develop a gene design algorithm for that host. Genes designed using GeneGPS reliably outperform genes designed using alternative methods.

The GeneGPS technology is protected by issued US patents 7561972, 7561973, 8126653, 8401798 and pending applications.

We have built GeneGPS optimization algorithms for many bacterial, yeast, insect, plant, fungal and mammalian expression hosts. We continue to develop GeneGPS for novel expression hosts.

Gene variants optimized by GeneGPS express up to 10-fold more protein in E. coli than variants created using competitors' algorithms (high CAI, matching host bias, etc.).

Vector Design
Powered by VectorGPS®

To optimize the gene but not the vector is to leave the job half done. Vector elements can improve protein solubility, reduce aggregation, direct proteins to the correct compartment, mediate genetic stability and contribute to host cell health, as well as increasing expression yield.

Our extensive collection of vector elements include promoters, terminators, genetic insulators, RNA processing sequences, secretion signal sequences, solubility and affinity tags, selectable markers and bacterial and eukaryotic origins of replication. With VectorGPS we combine these elements systematically, test the resulting vectors experimentally, and use machine learning analysis of the data to identify the best combinations of elements.

We have developed many ready-to-use expression vectors with superior performance in a wide range of hosts. We continue to apply VectorGPS to design custom vectors for novel hosts and applications.

ATUM expression vectors typically outperform commercially-available vectors (pCDNA3.4, pTT5) for the transient expression of GFP in HEK293 (v1, v2), ExpiCHO (v5, v6) and in both HEK293 and ExpiCHO (v3, v4).

Complete Construct

Coupling gene optimization and vector design gives you the greatest chances of obtaining a highly performing expression construct.

A 5-fold improvement in titer of herceptin in HEK293 delivered by combining vector and gene optimization.

host expertise

We design genes and vectors optimized for the most frequently used expression hosts. If you’re interested in a different expression host, we can work with you to develop a custom gene and vector design tool for optimized expression in your host.

Have a question?
Let's talk.

ATUM customer support scientists are available to discuss cloning strategies, gene design constraints, bioinformatics analyses, and other molecular biology/biotechnology concerns.

Call

Corporate Headquarters
(Newark, California)
+1 650 853 8347

European Office
(Basel, Switzerland)
+41 (0) 61 262 0212

Email

We generally reply within a few hours.

info@atum.bio

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