protein
services

Our protein services combine expression optimization, purification expertise and comprehensive analytical capabilities to ensure that your protein is made according to your precise specifications. Whether you need protein made in bacterial, yeast or mammalian hosts, we can help.

Quality

Activity and purity come first. Always. Our expertise in expression and purification combined with state-of-the-art analytics ensures that every protein that we produce is of the highest quality.

Scale

From 96-well plates to multiple liters, delivering up to 10's of grams of purified protein from bacterial, yeast and mammalian expression hosts.

Speed

DNA synthesis through to protein purification 'all under one roof' enables us to rapidly complete your orders, whatever the size, in as little as 4 weeks.

TYPES OF MOLECULES

Antibodies and antibody fragments
Bi-specifics and multivalents
Antigens
Membrane Proteins
Fusion Proteins
Enzymes
Challenging Proteins

TYPES OF
MOLECULES

Antibodies and antibody fragments
Bi-specifics and multivalents
Antigens
Membrane Proteins
Fusion Proteins
Enzymes
Challenging Proteins

protein_servicesAsset 6

Molecule Selection

Every year, we purify many thousands of different proteins. If your protein is challenging because of poor expression, low solubility or a tendency to aggregate, we can help.

Gene Design & Synthesis

Higher titers start with better genes. Just provide us with the amino acid sequence of the protein to be expressed. We will design a gene and clone it into a vector for expression in: Mammalian Bacterial Yeast

Expression & Purification

Whether you need micrograms of many different proteins for your discovery program, or grams of highly purified protein for animal studies, we have the production scale and purification strategy to match. Our cell cultures range from 96-well plates of 1ml cultures through shake flasks to bioreactors delivering grams of proteins. Expression testing/piloting, desalting, custom buffer formulations, and aliquoting are all available on request.

Typical Titers

Distribution of yields of recombinant human IgG1 antibody produced by ATUM between January and May 2018. Culture volume was 50-100 ml.

Titer in mg/L

Distribution of yields of recombinant mouse IgG2a antibody produced by ATUM between January and May 2018. Culture volume was 1000 ml for mouse IgG2a.

Titer in mg/L

Analytics

ATUM offers a range of analytical services to characterize the identity, purity, quantity and quality of your protein. Our tools include gel electrophoresis, HPLC (reverse phase, ion exchange and size exclusion), mass spectrometry, bio-layer interferometry (ForteBio Octet Red384 and HTX systems) and bacterial endotoxin testing. We can design an analytical package appropriate for your molecule and subsequent applications including IND filing and GLP toxicology studies.

Assays

ATUM has extensive experience in measuring functional properties of proteins. This can be binding of an antibody to its antigen, the activity of an enzyme under specific physical conditions or other properties that make the protein useful.

Deliverables

Your purified protein is shipped overnight frozen or on ice packs as requested. A comprehensive report for every analytical service performed is uploaded electronically to your account. Download sample analytical reports:

Have a question?
Let's talk.

ATUM customer support scientists are available to discuss cloning strategies, gene design constraints, bioinformatics analyses, and other molecular biology/biotechnology concerns.

Call

Corporate Headquarters
(Newark, California)
+1 650 853 8347

Email

We generally reply within a few hours.

info@atum.bio

Chat with us