Expression from pD2500 Leap-In Stable Vectors can be further increased by co-transfecting with one of our new transposases. Many pD2500 vectors already perform extremely well for expression of single proteins, even without transposase. Transposases are potent tools for eliminating yield reductions that can otherwise occur in stable cell lines containing IRES-based bicistronic constructs. Use in combination with our glutamine synthase or dihydrofolate reductase markers to boost antibody production even faster.
Leap-In Transposase Increases Protein Expression
*piggyBac® is a registered trademark of Transposagen, Inc.
Leap-In Transposase Increases Bicistronic Protein Expression
FACS Analysis: Cell Populations Shift From Poor to High Expression
- Protein expression
- Genome editing
The Leap-In Transposase is an engineered enzyme that catalyzes the integration of a transposon, your gene, into TTAT sites in the target genome. The technology enables a specified sequence to behave as a transposon, a mobile genetic element, which can efficiently transpose between vectors and chromosomes via a “cut and paste” mechanism. During transposition, the Leap-In Transposase recognizes transposon-specific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and moves the contents from the original sites and efficiently integrates them into TTAT chromosomal sites. Similar technologies report integration of up to 20 copies of the gene into unique locations in the genome 72 hours post transfection.
- Up to 20 stable copies of your gene integrated into your target genome 72 hours post transfection
- Stable pools created in 2 weeks, Cell-Lines 6 weeks
- Single Transfection
- Unlimited payload
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