ProteinPaintbox®

Synthetic non-Aequorea fluorescent and chromogenic proteins bring a world of color to your research.

ATUM’s synthetic fluorescent and chromogenic proteins (non-Aequorea) are an ideal source of protein coding sequences (genes) that can be amplified by PCR or easily excised using the flanking restriction sites and cloned into any other expression vector of choice. These vectors can also be used as expression vectors or as positive controls, and allow monitoring of inducible protein expression.

Features

Feature E. coli Mammalian Pichia S. cerevisiae
Fluorescent Proteins CFP, GFP,
OFP, RFP, YFP
CFP, GFP,
OFP, RFP
CFP, GFP,
OFP, RFP
CFP, GFP,
OFP, RFP
Chromogenic Proteins Blue, Magenta,
Orange, Pink,
Purple, Teal,
Violet
No Blue, Magenta,
Orange, Pink,
Purple
Magenta, Pink, Purple
Promoter T5 EF1α, CMV AOX1 TEF
Electra System® Yes Yes Yes Yes

Video Overview


Presentations, Posters and White Papers


Vector Selector

Overview

ATUM’s synthetic non-Aequorea fluorescent proteins are intended to be used as a source of different fluorescent protein coding sequences (genes) that can be amplified by PCR or easily excised using the flanking BsaI restriction sites and cloned into any other expression vector of choice. These vectors can also be used as expression vectors (T5 promoter) or as positive controls and allow monitoring of inducible protein expression.

Protein Paintbox Fluorescent

Expression shown in E. coli, proteins are also available for other host systems.


Order Fluorescent Proteins

For purified protein, please contact us at info@atum.bio or 1-650-853-8347.

Name Ex/Em Datasheet  Add to Cart
Rainbow_Circle Fluorescent Set (14 Proteins) Various
CindyLouCFP 400/495
FrostyCFP 400/495
PartridgeCFP 395/500
SnowCFP 400/495
TwinkleCFP 400/495
CratchitYFP 520/540
KringleYFP 520/542
MarleyYFP 520/535
MatchYFP 520/542
YetiYFP 518/546
CometGFP 395/515
DasherGFP 505/525
HollyGFP 500/515
IvyGFP 500/510
SimeonOFP 550/563
TinyTimOFP 550/563
YukonOFP 550/563
CayenneRFP 554/590
FresnoRFP 553/592
GuajilloRFP 554/584
PaprikaRFP 554/590
SantakaRFP 558/603
SerranoRFP 554/590
RudolphRFP 553/570
EiraCFP (BioBricks) 398/498
BlazeYFP (BioBricks) 515/534
JuniperGFP (BioBricks) 508/521
TannenRFP (BioBricks) 550/565

Fluorescent Protein Spectra & Expression Images

Fluorescent Protein Excitation Overlay

Fluorescent Protein Spectra Overlay Excitation Image  

Fluorescent Protein Emission Overlay

Fluorescent Protein Spectra Overlay Emission Normalized Image  
Fluorescent Protein Spectra BlazeYFP Fluorescent
  • Excitation 515nm, Emission 534nm
  • Mol. Wt. 29.2kDA, Length: 263aa
  • Expression shown above in E. coli
Fluorescent Protein Spectra CayanneRFP Fluorescent Protein Expression CayenneRFP
  • Excitation 554nm, Emission 590nm
  • Mol. Wt. 26.6kDA, Length: 237aa
  • Expression shown above in E. coli, also available in Mammalian vectors
Fluorescent Protein Spectra CindyLouCFP Fluorescent Protein Expression CindyLouCFP
  • Excitation 400nm, Emission 495nm
  • Mol. Wt. 26.0kDA, Length: 234aa
  • Expression shown above in E. coli
Fluorescent Protein Spectra CometGFP Fluorescent Protein Expression CometGFP
  • Excitation 400nm, Emission 515nm
  • Mol. Wt. 26.4kDA, Length: 238aa
  • Expression shown above in E. coli, also available in Mammalian vectors
  • Anti-CometGFP™ polyclonal rabbit Ab is available (Catalog #AB-02)
Fluorescent Protein Spectra CrachitYFP Fluorescent Protein Expression CratchitYFP
  • Excitation 520nm, Emission 545nm
  • Mol. Wt. 26.4kDA, Length: 237aa
Fluorescent Protein Spectra DasherGFP Fluorescent Protein Expression DasherGFP
  • Excitation 505nm, Emission 525nm
  • Mol. Wt. 26.6kDA, Length: 237aa
  • Expression shown above in E. coli, also available in Mammalian and Yeast vectors
Fluorescent Protein Spectra EiraCFP Fluorescent Protein Expression EiraCFP_BB
  • Excitation 398nm, Emission 498nm
  • Mol. Wt. 26.0kDA, Length: 233aa
  • Expression shown above in E. coli
Fluorescent Protein Spectra FresnoRFP Fluorescent Protein Expression FresnoRFP
  • Excitation 553nm, Emission 592nm
  • Mol. Wt. 26.0kDA, Length: 233aa
  • Expression shown above in E. coli, also available in Mammalian and Yeast vectors
Fluorescent Protein Spectra GuajilloRFP Fluorescent Protein Expression GuajilloRFP
  • Excitation 554nm, Emission 584nm
  • Mol. Wt. 28.9kDA, Length: 263aa
  • Expression shown above in E. coli
Fluorescent Protein Spectra FrostyCFP Fluorescent Protein Expression FrostyCFP
  • Excitation 400nm, Emission 495nm
  • Mol. Wt. 26.5kDA, Length: 237aa
Fluorescent Protein Spectra HollyGFP Fluorescent Protein Expression HollyGFP
  • Excitation 500nm, Emission 515nm
  • Mol. Wt. 26.0kDA, Length: 234aa
Fluorescent Protein Spectra IvyGFP Fluorescent Protein Expression IvyGFP
  • Excitation 400nm, Emission 515nm
  • Mol. Wt. 24.9kDA, Length: 222aa
Fluorescent Protein Expression JuniperGFP_BB
  • Excitation 508nm, Emission 521nm
  • Mol. Wt. 26.6kDA, Length: 237aa
  • Expression shown above in E. coli
Fluorescent Protein Spectra KringleYFP Fluorescent Protein Expression KringleYFP
  • Excitation 520nm, Emission 542nm
  • Mol. Wt. 26.4kDA, Length: 237aa
  • Expression shown above in E. coli, also available in Yeast vectors
Fluorescent Protein Spectra MarleyYFP Fluorescent Protein Expression MarleyYFP
  • Excitation 520nm, Emission 538nm
  • Mol. Wt. 26.4kDA, Length: 237aa
  • Expression shown above in E. coli, also available in Yeast vectors
Fluorescent Protein Spectra PaprikaRFP Fluorescent Protein Expression PaprikaRFP
  • Excitation 554nm, Emission 590nm
  • Mol. Wt. 29.2kDA, Length: 263aa
  • Expression shown above in E. coli, also available in Mammalian and Yeast vectors
Fluorescent Protein Spectra RudolphRFP Fluorescent Protein Expression RudolphRFP
  • Excitation 553nm, Emission 570nm
  • Mol. Wt. 25.3kDA, Length: 227aa
  • Expression shown above in E. coli, also available in Mammalian and Yeast vectors
Fluorescent Protein Spectra SantakaRFP Fluorescent Protein Expression SantakaRFP
  • Excitation 558nm, Emission 603nm
  • Mol. Wt. 29.0kDA, Length: 263aa
  • Expression shown above in E. coli, also available in Mammalian and Yeast vectors
Fluorescent Protein Spectra SerranoRFP Fluorescent Protein Expression SerranoRFP
  • Excitation 554nm, Emission 590nm
  • Mol. Wt. 26.5kDA, Length: 237aa
  • Expression shown above in E. coli, also available in Mammalian and Yeast vectors
Fluorescent Protein Spectra TannenRFP Fluorescent Protein Expression TannenRFP
  • Excitation 550nm, Emission 565nm
  • Mol. Wt. 25.2kDA, Length: 227aa
Fluorescent Protein Spectra TwinkleCFP Fluorescent Protein Expression TwinkleCFP
  • Excitation 400nm, Emission 495nm
  • Mol. Wt. 26.1kDA, Length: 234aa
  • Expression shown above in E. coli, also available in Mammalian vectors
Fluorescent Protein Spectra YetiYFP Fluorescent Protein Expression YetiYFP
  • Excitation 518nm, Emission 546nm
  • Mol. Wt. 26.4kDA, Length: 237aa
Fluorescent Protein Spectra YukonOFP Fluorescent Protein Expression YukonOFP
  • Excitation 550nm, Emission 563nm
  • Mol. Wt. 25.4kDA, Length: 227aa
  • Expression shown above in E. coli, also available in Mammalian vectors

Overview

ATUM’s chromogenic proteins are intended to be used as a source of different color protein coding sequences (genes) that can be amplified by PCR or easily excised using the flanking BsaI restriction sites and cloned into any other expression vector of choice. These vectors can also be used as expression vectors (T5 promoter) or as positive controls and allow monitoring of inducible protein expression.

Chromogenic Protein Paintbox all colors

Expression shown in E. coli, proteins are also available for other host systems.


Order Chromogenic Proteins

For purified protein, please contact us at info@atum.bio or 1-650-853-8347.

Name Datasheet  Add to Cart
Rainbow_Circle_Chromogenic Chromogenic Set (12 Proteins)
BlitzenBlue
DreidelTeal
VirginiaViolet
VixenPurple
PrancerPurple®
TinselPurple
MaccabeePurple
DonnerMagenta
CupidPink
LailaPink
PaxPink
SeraphinaPink
ScroogeOrange
LeorOrange

Yes!
The individual color names in the ProteinPaintbox derive from our 2011 Holiday publication, Nine improved monomeric fluorescent proteins from Rangifer tarandus, where the IP-Free proteins were named after Santa’s reindeer (Rudolph, Donner, Comet, etc…). As the ATUM fluorescent and chromogenic proteins became available for sale, we kept the reindeer names. As we added new colors to our product line, we continued with the holiday naming theme, branching out to include a diversity of cultural winter traditions, classic holiday literature, drama, and of course, television cartoons. We hope you enjoy this naming scheme.
Should you have any questions regarding the protein names or wish to suggest additional names, please email us at communications@atum.bio.
Sure, you can learn more about vector design with T5 promoter, lac operator for IPTG induction and increased protein expression in bacterial systems here.
Multiple CFPs, GFPs, etc. have been selected to cover a range of intensities. For example, FrostyCFP – 20000, CindyLouCFP – 35000, TwinkleCFP – 12000. It is also important to remember, that while optimized for each host system, these protein sequences have not been optimized for your individual project needs. By providing you with a large range of choices, you can test a variety of proteins to determine which works best for your research.
No, the Paintbox proteins need oxygen to mature, so cannot be expressed anaerobically.
The Biobrick set (3 FPs, EiraCFP, JuniperGFP and BlazeYFP) have been donated by ATUM to the BioBricks Foundation and are covered under the Biobricks licensing agreement (BLA). These belong to BioBricks, and are therefore not included in the ATUM ProteinPaintbox set.
The green/yellow FPs share only 27% homology at the amino acid level. Thus, it is fair to say that they are all unique proteins.
In the ATUM labs, we get colored colonies in 24hrs.  As for how fast the maturation is, we have not done a time-course analysis. So, we cannot say if color develops in an hour or if it takes longer. Maturation time at 37°C – 24 hrs incubation is fast, 24-72 hrs is medium.
ATUM offers an antibody (anti-CometGFP, catalog #AB-01) that will detect GFP which could be used to determine the number of GFP molecules. However, we do not currently have data for this. Note, since this is a polyclonal Ab, it should be able to detect any of our GFPs.
Maturation time and intensity varies for each of the proteins.
For example, the intensity of RudolphRFP is lower than DasherGFP, while maturation time for RudolphRFP is longer than for DasherGFP. Thus at an early timepoint, you may have the appearance of excellent DasherGFP expression, while only minimal RudolphRFP expression.
We have not measured half-life.
However, we have looked at color after a week and it appears to remain stable.
These proteins should be able to express in any genetic background that will support T5 driven expression.
Yes, they should mature in minimal media induced with IPTG.

Let ATUM Increase Your Expression

Consult with an Expression Specialist today at +1 877 362 8646 or info@atum.bio to achieve your research goals quickly and affordably