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Learn more about ATUM Products and Services. Webinars are applicable for new and experienced researchers alike and can be viewed in any order, singly or as a group.

 

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CRISPR/Cas9 Technology

  • A brief overview on CRISPR Cas9 Technology and its applications
  • CRISPR products available in different host systems
  • Information and a demo of gRNA design tool
  • Technologies developed to minimize off-target effects
  • gRNA libraries

Clustered Regularly Interspaced Short Palindromic Repeats, or CRISPR, is an attractive next-generation genome-engineering tool due to its ease of use and high efficiency in a range of host systems. This exciting technology enables precise genome editing, including gene knock-outs and knock-ins using a Cas9 nuclease and targeting short guide RNA (sgRNA). ATUM has developed a series of solutions to suppress off-target effects by controlling Cas9 expression and developing a gRNA design bioinformatics tool. Furthermore, we have developed the NickaseNinja™ vector which offers high fidelity in a convenient, single-vector co-expressing two tandem sgRNAs using dual RNA polymerase promoters (patent pending). In addition, an emerging application using sgRNA libraries with a lenti-viral delivery mechanism to provide means of large-scale, loss-of-function screens will be further discussed.

View a pdf of the webinar slides.


Protein Engineering via Systematic Exploration of Sequence Space

Learn more about Protein Engineering and Infologs. ProteinGPS relies on identifying key amino acid substitutions through bioinformatics-based mining of available sequence space and combining such substitutions in information maximized Infologs – synthetic gene variants designed to be systematically varied across the searched space.

Using a small set of variants to explore the sequence space systematically can help us understand the effects of substitutions on the protein activities and further helps us to determine e strategies to explore the sequence space. This can be attained using Machine Learning techniques to analyze the data from a small number of systematically designed variants of the protein, usually on the order of 100 variants. We can address questions related to additivity and multidimensional effects of substitutions on the various properties and activities that can be measured accurately under commercially relevant conditions.

Learn more about the following ProteinGPS Advantages:

  • Optimize directly for function in the final application
  • Save years of time and millions of dollars
  • No high-throughput (HTP) screens
  • Screen small numbers of variants (50-200) directly for the desired function, ideal for Enzyme Engineering
  • Don’t waste time pursuing false positives: variants identified by HTP screens that do not retain activity in ‘real’ assay
  • No false negatives lost due to screening error or poor correlation between HTP screen and ‘real’ assay

View a pdf of the webinar slides.


Mammalian Expression Vectors & Panels

Learn more about Mammalian Expression Vectors & Panels and how these can benefit your research. ATUM offers a large variety of expression vectors. Some vector properties are uniquely suited to different applications, and vector diversity is useful because proteins do not all behave in the same way. Our vectors are designed to maximize the chances that at least one will have the expression properties you need.

  • A brief overview on Mammalian host systems and expression
  • Discussion of Transient, Stable and Lentivirus expression
  • In-depth information on how to select the best vector for your system and research
  • Information and a demo of our Vector Selector
  • Information on our technologies developed to provide Expression Monitoring, Localization Monitoring and increased Protein Expression

View a pdf of the webinar slides.


Electra Vector System®

Learn more about the Electra Vector System® and how it can benefit your research. ATUM has developed a simple one-tube universal cloning process that can be performed in a 5 minute bench-top reaction with the fidelity of a restriction based cloning system. The system includes a collection of IP-Free bacterial, mammalian and yeast expression vectors that provide a quick and efficient way to test a gene of interest under control of various elements and are available with optional C- and N-terminal tags and/or fusions. Any vector can be easily “Electra-fied” (converted to function as an Electra vector), and ATUM will assist anyone who wishes to do so.

View a pdf of the webinar slides.


Gene Designer – A Powerful Bioinformatics Tool for Genetic Engineering

Developed by ATUM, Gene Designer 2.0 is the ideal software to design sequences de novo without being limited by what nature can provide. It encompasses a complete and visually rich set of tools to bring your creative ideas to completion. We demonstrate how to design gene sequences as well as how to remove or add restriction sites or other motifs, primer design, cloning scheme, and many other features. March 8, 2012
Gene Designer software is available free of charge.


Optimizing Gene Expression and Performance using GeneGPS™

Gene synthesis allows researchers to tailor gene sequences for optimal utility for any application. However, the relationship between gene sequence and expression is complex and depends on a wide range of sequence variables. ATUM uses a systematic engineering approach, GeneGPS, to navigate these variables with a minimal number of test genes to optimize performance. Application of this approach with a variety of protein targets and expression hosts will be described. March 22, 2012
View a pdf of the webinar slides.


Using ProteinGPS and Infologs to Engineer Biological Systems

ProteinGPS™ relies on identifying key amino acid substitutions through bioinformatics-based mining of available sequence space and combining such substitutions in information maximized Infologs – synthetic gene variants designed to be systematically varied across the searched space. The presentation includes recent case studies. April 19, 2012
View a pdf of the webinar slides.


Aldevron Webinar – Optimizing Gene Expression/Performance using GeneGPS™

Gene synthesis allows researchers to tailor gene sequences for optimal utility for any application. However, the relationship between gene sequence and expression is complex and depends on a wide range of sequence variables. ATUM uses a systematic engineering approach, GeneGPS, to navigate these variables with a minimal number of test genes to optimize performance. Application of this approach with a variety of protein targets and expression hosts is described.
View a pdf of the ATUM webinar slides.


AAAS Science Webinar – From Genes to Proteins: the Impact of Gene Sequence on Translation and Expression

Archive of a webinar by ATUM and the AAAS, originally presented in October 2009. This webinar includes presentations by Joshua Plotkin, Ph.D., University of Pennsylvania; Christine Vogel, Ph.D., University of Texas at Austin; and Mark Welch, Ph.D., ATUM. October, 2009

 

 



Synthetic Biology Solutions – ATUM Overview


CRISPR/Cas9 Overview


CRISPR gRNA Design Tool Demo


ProteinPaintbox Overview


GeneGPS Optimization Overview


ProteinGPS Overview