CRISPR gRNA Design tool

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1. Select Target

Gene

Name

Gene

Locus

Heterologous

DNA

2. Results

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gRNA

3. Delivery Options

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Vector

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Oligos

CRISPR gRNA Design tool

Design my gRNA
I have my own gRNA

Species

PAM

I want

Wild-type Cas9

Nickase (Recommended)

Order your tandem gRNAs in the All-in-one NickaseNinja™ construct for increased transfection efficiency.

gRNA offset

Target a gene

Gene Name:

Search only in the first common exon

Target a genomic region

Chromosome:

Start Base:

End Base:

Target a sequence

ACGT only. Maximum of 10,000 bp.

Non-encoding/special characters

Species:

Enter your gRNA in FASTA format:

Non-encoding/special characters SapI restriction site

This tool currently accepts 20 bp gRNA's for cloning into a Cas9 vector. If you would like to use ATUM's NickaseNinja™ vector with tandem custom gRNAs, please email us at info@atum.bio

**Disclaimer**:

The ATUM gRNA Design Tool is provided "as is", with no explicit or implicit guarantees for any purpose. ATUM cannot guarantee the performance of any individual gRNA designed using the Tool. Please see our Terms & Conditions for additional information and complete disclaimer language. Thank you.

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