CRISPR gRNA Design tool

• Design my gRNA
• I have my own gRNA

Species:

Homo sapiens (GRCh37.p13) Mus musculus (GRCm38.p2) Saccharomyces cerevisiae S288C (R64-1-1) Escherichia coli K12 MG1655 (ASM584v2) Arabidopsis thaliana (TAIR10)  

PAM:

NGG NGG or NAG  

I want:

Wild-type Cas9  Nickase (Recommended)  Order your tandem gRNAs in the All-in-one NickaseNinja™ construct for increased transfection efficiency.

gRNA offset:

to  

Target a gene

Gene Name:

Show me an example

Search only in the first common exon

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Target a genomic region

Chromosome:

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 X Y Show me an example

Start Base:

End Base:

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Target a sequence

ACGT only. Maximum of 10,000 bp. Show me an example

Non-encoding/special characters

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Species:

Homo sapiens (GRCh37.p13) Mus musculus (GRCm38.p2)  

Enter your gRNA in FASTA format:

Non-encoding/special characters  SapI restriction site

This tool currently accepts 20 bp gRNA's for cloning into a Cas9 vector. If you would like to use ATUM's NickaseNinja™ vector with tandem custom gRNAs, please email us at info@atum.bio

Continue

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Disclaimer: The ATUM gRNA Design Tool is provided "as is", with no explicit or implicit guarantees for any purpose. ATUM cannot guarantee the performance of any individual gRNA designed using the Tool. Please see our Terms & Conditions for additional information and complete disclaimer language. Thank you.