Monitor inducible protein expression with ATUM's fluorescent and chromogenic proteins.
ATUM's fluorescent and chromogenic proteins are an ideal source of protein coding sequences
that can be quickly cloned into any expression vector of choice.
ATUM's fluorescent and chromogenic proteins cloned into expression vectors can be used as positive controls.
Overview & Order
Order & Expression Images
Synthetic non-Aequorea fluorescent and chromogenic proteins
bring a world of color to your research.
ATUM's synthetic fluorescent and chromogenic proteins (non-Aequorea)
are an ideal source of protein coding sequences (genes) that can be
easily excised using the flanking restriction sites and cloned into
any other expression vector of choice. These vectors can also be used
as expression vectors or as positive controls, and allow monitoring
of inducible protein expression.
U.S. Patent Nos. 8,975,042; 9,290,552; 9,493,521 and 9,771,402. Fluorescent and colored proteins and methods for using them. Minshull and Theodorou
Fluorescent Proteins - Overview
ATUM’s synthetic non-Aequorea fluorescent proteins are intended to be used as a source
of different fluorescent protein coding sequences (genes) that can be easily excised
using the flanking BsaI restriction sites and cloned into any other expression vector
of choice. These vectors can also be used as expression vectors (T5 promoter) or as
positive controls and allow monitoring of inducible protein expression.
ATUM's chromogenic proteins are intended to be used as a source of different
color protein coding sequences (genes) that can be easily excised using the
flanking BsaI restriction sites and cloned into any other expression vector
of choice. These vectors can also be used as expression vectors (T5 promoter)
or as positive controls and allow monitoring of inducible protein expression.
Yes! The individual color names in the ProteinPaintbox derive from our 2011 Holiday
publication, Nine improved monomeric fluorescent proteins from Rangifer tarandus,
where the IP-Free proteins were named after Santa’s reindeer (Rudolph, Donner,
Comet, etc…). As the ATUM fluorescent and chromogenic proteins became available
for sale, we kept the reindeer names. As we added new colors to our product line,
we continued with the holiday naming theme, branching out to include a diversity
of cultural winter traditions, classic holiday literature, drama, and of course,
television cartoons. We hope you enjoy this naming scheme.
Should you have any questions regarding the protein names or wish to suggest
additional names, please email us at firstname.lastname@example.org.
Can you tell me more about the T5 promoter?
Sure, you can learn more about vector design with T5 promoter, lac operator for IPTG
induction and increased protein expression in bacterial systems here.
What volume is provided?
2µg lyophilized plasmid
Why are there so many?
Multiple CFPs, GFPs, etc. have been selected to cover a range of intensities.
For example, FrostyCFP – 20000, CindyLouCFP – 35000, TwinkleCFP – 12000. It is
also important to remember, that while optimized for each host system, these
protein sequences have not been optimized for your individual project needs.
By providing you with a large range of choices, you can test a variety of
proteins to determine which works best for your research.
Can Paintbox Proteins express in anaerobic systems?
No, the Paintbox proteins need oxygen to mature, so cannot be expressed anaerobically.
Why are the Biobrick FPs not included in your Paintbox?
The Biobrick set (3 FPs, EiraCFP, JuniperGFP and BlazeYFP) have been donated by
ATUM to the BioBricks Foundation and are covered under the Biobricks licensing
agreement (BLA). These belong to BioBricks, and are therefore not included in
the ATUM ProteinPaintbox set.
Are these unique proteins? Or these essentially derivatives or mutants of a
The green/yellow FPs share only 27% homology at the amino acid level. Thus, it
is fair to say that they are all unique proteins.
How long is the maturation time?
In the ATUM labs, we get colored colonies in 24hrs. As for how fast the
maturation is, we have not done a time-course analysis. So, we cannot say
if color develops in an hour or if it takes longer. Maturation time at
37°C – 24 hrs incubation is fast, 24-72 hrs is medium.
Do you have a simple method to measure how many GFP molecules are
ATUM offers an antibody (anti-CometGFP, catalog #AB-01) that will detect
GFP which could be used to determine the number of GFP molecules. However,
we do not currently have data for this. Note, since this is a polyclonal
Ab, it should be able to detect any of our GFPs.
Why can I detect one protein but not another?
Maturation time and intensity varies for each of the proteins.
For example, the intensity of RudolphRFP is lower than DasherGFP, while
maturation time for RudolphRFP is longer than for DasherGFP. Thus at an early
timepoint, you may have the appearance of excellent DasherGFP expression,
while only minimal RudolphRFP expression.
What is the half-life of these proteins?
We have not measured half-life.
However, we have looked at color after a week and it appears to remain stable.
Do the proteins require any particular genetic background to mature?
These proteins should be able to express in any genetic background that
will support T5 driven expression.
Can the proteins mature in minimal media?
Yes, they should mature in minimal media induced with IPTG.
Have a question? Let's talk.
ATUM customer support scientists are available to discuss cloning strategies,
gene design constraints, bioinformatics analyses, and other molecular