Protein Paintbox

Yield

Monitor inducible protein expression with ATUM's fluorescent and chromogenic proteins.

Speed

ATUM's fluorescent and chromogenic proteins are an ideal source of protein coding sequences that can be quickly cloned into any expression vector of choice.

Precision

ATUM's fluorescent and chromogenic proteins cloned into expression vectors can be used as positive controls.

Protein Paintbox

ProteinPaintbox®

Synthetic non-Aequorea fluorescent and chromogenic proteins bring a world of color to your research.

Overview

ATUM's synthetic fluorescent and chromogenic proteins (non-Aequorea) are an ideal source of protein coding sequences (genes) that can be easily excised using the flanking restriction sites and cloned into any other expression vector of choice. These vectors can also be used as expression vectors or as positive controls, and allow monitoring of inducible protein expression.

Features

Feature E. coli Mammalian Pichia S. cerevisiae
Fluorescent Proteins CFP, GFP, OFP, RFP, YFP CFP, GFP, OFP, RFP CFP, GFP, OFP, RFP CFP, GFP, OFP, RFP
Chromogenic Proteins Blue, Magenta, Orange, Pink, Purple, Teal, Violet No Blue, Magenta, Orange, Pink, Purple Magenta, Pink, Purple
Promoter T5 EF1α, CMV AOX1 TEF
Electra System® Yes Yes Yes Yes

Presentations, Posters and Whitepapers

Fluorescent Proteins - Overview

ATUM’s synthetic non-Aequorea fluorescent proteins are intended to be used as a source of different fluorescent protein coding sequences (genes) that can be easily excised using the flanking BsaI restriction sites and cloned into any other expression vector of choice. These vectors can also be used as expression vectors (T5 promoter) or as positive controls and allow monitoring of inducible protein expression.

Order Fluorescent Proteins

Name Ex/Em Datasheet Add to Cart
Various
400/495
400/495
PartridgeCFP
395/500
SnowCFP
400/495
400/495
520/540
520/542
520/535
MatchYFP
520/542
518/546
395/515
505/525
500/515
500/510
SimeonOFP
550/563
TinyTimOFP
550/563
550/563
554/590
553/592
554/584
554/590
558/603
554/590
553/570
EiraCFP (BioBricks)
398/498
515/534
508/521
550/565

Fluorescent Protein Excitation Overlay

Fluorescent Protein Emission Overlay

Excitation 400nm, Emission 495nm

Mol. Wt. 26.0kDA, Length: 234aa

Expression shown above in E. coli

Excitation 400nm, Emission 495nm

Mol. Wt. 26.5kDA, Length: 237aa

Expression shown above in E. coli

Excitation 400nm, Emission 495nm

Mol. Wt. 26.1kDA, Length: 234aa

Expression shown above in E. coli, also available in Mammalian vectors

Excitation 520nm, Emission 545nm

Mol. Wt. 26.4kDA, Length: 237aa

Expression shown above in E. coli

Excitation 520nm, Emission 542nm

Mol. Wt. 26.4kDA, Length: 237aa

Expression shown above in E. coli, also available in Yeast vectors

Excitation 520nm, Emission 538nm

Mol. Wt. 26.4kDA, Length: 237aa

Expression shown above in E. coli, also available in Yeast vectors

Excitation 518nm, Emission 546nm

Mol. Wt. 26.4kDA, Length: 237aa

Expression shown above in E. coli

Excitation 400nm, Emission 515nm

Mol. Wt. 26.4kDA, Length: 238aa

Expression shown above in E. coli, also available in Mammalian vectors

Anti-CometGFP™ polyclonal rabbit Ab is available (Catalog #AB-02)

Excitation 505nm, Emission 525nm

Mol. Wt. 26.6kDA, Length: 237aa

Expression shown above in E. coli, also available in Mammalian and Yeast vectors

Excitation 500nm, Emission 515nm

Mol. Wt. 26.0kDA, Length: 234aa

Expression shown above in E. coli

Excitation 400nm, Emission 515nm

Mol. Wt. 24.9kDA, Length: 222aa

Expression shown above in E. coli

Excitation 550nm, Emission 563nm

Mol. Wt. 25.4kDA, Length: 227aa

Expression shown above in E. coli, also available in Mammalian vectors

Excitation 554nm, Emission 590nm

Mol. Wt. 26.6kDA, Length: 237aa

Expression shown above in E. coli, also available in Mammalian vectors

Excitation 553nm, Emission 592nm

Mol. Wt. 26.0kDA, Length: 233aa

Expression shown above in E. coli, also available in Mammalian and Yeast vectors

Excitation 554nm, Emission 584nm

Mol. Wt. 28.9kDA, Length: 263aa

Expression shown above in E. coli

Excitation 554nm, Emission 590nm

Mol. Wt. 29.2kDA, Length: 263aa

Expression shown above in E. coli, also available in Mammalian and Yeast vectors

Excitation 558nm, Emission 603nm

Mol. Wt. 29.0kDA, Length: 263aa

Expression shown above in E. coli, also available in Mammalian and Yeast vectors

Excitation 554nm, Emission 590nm

Mol. Wt. 26.5kDA, Length: 237aa

Expression shown above in E. coli, also available in Mammalian and Yeast vectors

Excitation 553nm, Emission 570nm

Mol. Wt. 25.3kDA, Length: 227aa

Expression shown above in E. coli, also available in Mammalian and Yeast vectors

Excitation 515nm, Emission 534nm

Mol. Wt. 29.2kDA, Length: 263aa

Expression shown above in E. coli

Excitation 508nm, Emission 521nm

Mol. Wt. 26.6kDA, Length: 237aa

Expression shown above in E. coli

Excitation 550nm, Emission 565nm

Mol. Wt. 25.2kDA, Length: 227aa

Expression shown above in E. coli

Chromogenic Proteins - Overview

ATUM's chromogenic proteins are intended to be used as a source of different color protein coding sequences (genes) that can be easily excised using the flanking BsaI restriction sites and cloned into any other expression vector of choice. These vectors can also be used as expression vectors (T5 promoter) or as positive controls and allow monitoring of inducible protein expression.

Order Chromogenic Proteins

Name Datasheet Add to Cart
Chromogenic Set (12 Proteins)
LailaPink
PaxPink

FAQ

What are the terms of use?

See the complete legal Terms and Conditions.

Can you explain the protein names?

Yes! The individual color names in the ProteinPaintbox derive from our 2011 Holiday publication, Nine improved monomeric fluorescent proteins from Rangifer tarandus, where the IP-Free proteins were named after Santa’s reindeer (Rudolph, Donner, Comet, etc…). As the ATUM fluorescent and chromogenic proteins became available for sale, we kept the reindeer names. As we added new colors to our product line, we continued with the holiday naming theme, branching out to include a diversity of cultural winter traditions, classic holiday literature, drama, and of course, television cartoons. We hope you enjoy this naming scheme. Should you have any questions regarding the protein names or wish to suggest additional names, please email us at communications@atum.bio.

Can you tell me more about the T5 promoter?

Sure, you can learn more about vector design with T5 promoter, lac operator for IPTG induction and increased protein expression in bacterial systems here.

What volume is provided?

2µg lyophilized plasmid

Why are there so many?

Multiple CFPs, GFPs, etc. have been selected to cover a range of intensities. For example, FrostyCFP – 20000, CindyLouCFP – 35000, TwinkleCFP – 12000. It is also important to remember, that while optimized for each host system, these protein sequences have not been optimized for your individual project needs. By providing you with a large range of choices, you can test a variety of proteins to determine which works best for your research.

Can Paintbox Proteins express in anaerobic systems?

No, the Paintbox proteins need oxygen to mature, so cannot be expressed anaerobically.

Why are the Biobrick FPs not included in your Paintbox?

The Biobrick set (3 FPs, EiraCFP, JuniperGFP and BlazeYFP) have been donated by ATUM to the BioBricks Foundation and are covered under the Biobricks licensing agreement (BLA). These belong to BioBricks, and are therefore not included in the ATUM ProteinPaintbox set.

Are these unique proteins? Or these essentially derivatives or mutants of a 'base' protein?

The green/yellow FPs share only 27% homology at the amino acid level. Thus, it is fair to say that they are all unique proteins.

How long is the maturation time?

In the ATUM labs, we get colored colonies in 24hrs. As for how fast the maturation is, we have not done a time-course analysis. So, we cannot say if color develops in an hour or if it takes longer. Maturation time at 37°C – 24 hrs incubation is fast, 24-72 hrs is medium.

Do you have a simple method to measure how many GFP molecules are expressed?

ATUM offers an antibody (anti-CometGFP, catalog #AB-01) that will detect GFP which could be used to determine the number of GFP molecules. However, we do not currently have data for this. Note, since this is a polyclonal Ab, it should be able to detect any of our GFPs.

Why can I detect one protein but not another?

Maturation time and intensity varies for each of the proteins.

For example, the intensity of RudolphRFP is lower than DasherGFP, while maturation time for RudolphRFP is longer than for DasherGFP. Thus at an early timepoint, you may have the appearance of excellent DasherGFP expression, while only minimal RudolphRFP expression.

What is the half-life of these proteins?

We have not measured half-life.

However, we have looked at color after a week and it appears to remain stable.

Do the proteins require any particular genetic background to mature?

These proteins should be able to express in any genetic background that will support T5 driven expression.

Can the proteins mature in minimal media?

Yes, they should mature in minimal media induced with IPTG.

Have a question?
Let's talk.

ATUM customer support scientists are available to discuss cloning strategies, gene design constraints, bioinformatics analyses, and other molecular biology/biotechnology concerns.

Call

Corporate Headquarters
(Newark, California)
+1 650 853 8347

European Office
(Basel, Switzerland)
+41 (0) 61 262 0212

Email

We generally reply within a few hours.

info@atum.bio

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